Rob and Carole Hughes set up Anna's Hope in memory of their daughter to help children and teenagers suffering from brain tumours.
Anna was diagnosed with a brain tumour in 2004. She remained full of life despite months of chemotherapy but lost her battle aged just three.
It was a proud moment on Tuesday for the couple when they announced Alison Mitchell, an additional paediatric neuro-oncology nurse specialist, will be providing a vital lifeline to other families.
The three-year post at Addenbrookes has been funded by a £150,000 donation from the charity.
Alison will be based at the hospital but will be working alongside Anna's nurse Sharon Taylor in providing support to hundreds of families across the East Midlands.
But Rob, 52, and Carole, 47, of Pilsgate, near Stamford, are not planning to stop there as they aim to provide an Anna's Hope nurse in every region in the country.
They set out in 2006 to raise £1m and say that is still the aim.
People across Stamford and the surrounding area have backed the campaign with a variety of fundraising events including sponsored bike rides and walks, raffles, a spring fair, charity balls and collections.
Rob said: "I feel proud for Anna that she inspired us to do something in her name and proud for all the people that have helped us.
"The public, businesses and schools all should feel proud as they have helped us to make a real difference.
"We know that having someone there for you when you need them takes a lot of the burden off your shoulders so you can concentrate on your child.
"When Anna died we were determined that something positive should come out of our own personal tragedy. Anna's nurse was such a tower of strength to us throughout her illness."
Rob says Sharon, known as Harry, was with the family all the way through Anna's 15-month illness working tirelessly.
Rob, who himself cycled from Land's End to John O'Groats, said: "She was also there during Anna's final days co-ordinating the local doctors and nurses so that Anna could be with her mummy, daddy and sister Sara without pain at home."
Carole said: "We wish to say a special thank you to all the people, schools and local businesses. Without them we would not have been able to fund the specialist nurse giving hope to all these children which they certainly deserve."
Brain tumours are the biggest cancer killer of children and young people with more than 350 children diagnosed each year in the UK, resulting in more than 100 deaths.
Addenbrookes clinical director of children's services, Dr Denise Williams said: "We know this will make a real difference to the co-ordination of care and support for families at every stage of treatment."
The charity has so far raised £250,000 and the remaining £100,000 will go towards more rehabilitation support at Addenbrookes and its £1m target.

ALISON'S EAGER TO START HELPING
Alison, who lives with husband Adam Pearson in Radcliffe Road, Stamford, is excited to be providing help to hundreds of families thanks to fundraising.
She said: "It is a much needed job as being diagnosed with a brain tumour is very scary for both the children and the parents and they need a lot of support and information.
"It is quite a privilege to be involved with these families.
"I think it is amazing how much people can do when they put their minds to it and how generous everyone, especially in the Stamford area, has been."
Alison, 32, has been working as a paediatric oncology nurse during her 10-year nursing career.
She qualified in Edinburgh and gained experience by working in Birmingham and London.
Both Alison and nurse Sharon Taylor support the families along with co-ordinating the patients' care and liaising between Addenbrookes and other hospitals.
The nurses will be covering a large area which covers the whole of East Anglia, including Lincolnshire and Bedford.